A new system to evaluate characteristics of Niemann-Pick C1 Like 1-mediated cholesterol transport using Xenopus laevis oocytes

Niemann-Pick C1 Like 1 (NPC1L1) is known to be involved in the intestinal absorption of cholesterol. For evaluating function NPC1L1, cell lines such as Caco-2, Madin-Darby canine kidney (MDCK) II, and McA-RH7777 have been used previous studies, but detailed molecular mechanism transport has not elucidated. In this study, characteristics cholesterol via NPC1L1 were investigated using a Xenopus laevis oocyte expression system addition conventional line with stable expression. The activity uptake was increased NPC1L1-overexpressed MDCK cells compared that mock cells, expressed endogenous had high activity. On other hand, cRNA-injected oocytes after culturing for 5–6 days. water-injected oocytes. addition, decreased presence ezetimibe, an inhibitor, control oocytes, indicating Furthermore, substantially L216A leucine at position 216 important useful mutant analysis. These results indicate NPC1L1-mediated may contribute elucidation NPC1L1. • An established. lower also elucidated by system.